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微信小章| 产品名称： | Human Coxsackievirus A 9 |
|---|---|
| 商品货号： | TS196079 |
| Classification： | Picornaviridae, Enterovirus, Human enterovirus B |
| Deposited As： | Coxsackievirus A9 |
| Agent： | Human Coxsackievirus A 9 |
| Strain： | P.B. (Bozek) |
| Applications： | No CPE produced in HeLa cells. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Following reconstitution, dilute lyophilized material at least 1:10 in physiological culture medium prior to infecting cells in vitro. No CPE produced in HeLa cells. |
| Effect on Host： | CPE, complete degeneration of cells |
| Recommended Host： | LLC-MK2 cells (ATCC® CCL-7™) |
| Growth Conditions： | Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent). Incubation: 2 to 5 days at 37°C, a 5% CO2 in air atmosphere is recommended. |
| Name of Depositor： | HA Wenner |
| Source： | Stool from 6-year-old child, New York, 1950 |
| Year of Origin： | 1950 |
| References： | Dalldorf G. The coxsackie virus group. Ann. N.Y. Acad. Sci. 56: 583-586, 1953. PubMed: 13139263 Robbins FC, et al. Studies on the cultivation of poliomyelitis viruses in tissue culture. V. The direct isolation and serologic identification of virus strains in tissue culture from patients with nonparalytic and paralytic poliomyelitis. Am. J. Hyg. 54: 286-293, 1951. PubMed: 14877809 |
| Cross References： | Nucleotide (GenBank) : AJ295200 Human coxsackievirus A9B genomic RNA for partial polyprotein gene, isolate VR-186, ATCC. |