Yes

1. Harvest cells from a culture which is at or near peak density by centrifugation at ~800 x g for 10 min.
2. Adjust concentration of cells to 2 x 10⁷ - 2 x 10⁸ mL in fresh growth medium. xa0If the concentration is too low, centrifuge at ~800 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired cell concentration.
3. While cells are centrifuging, prepare a 10% (v/v) solution of sterile methanol in fresh growth medium.xa0
4. Mix the cell suspension and the methanol solution in equal portions. The final concentration will be 10⁷ – 10⁸ cells/mL and 5% (v/v) of methanol. The time from the mixing of the cell preparation and methanol stock solution before the freezing process is begun should be no less than 5 min and no more than 15 min.
5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryovials.
6. Place the vials in a controlled rate freezing unit.xa0 From room temperature cool at -1°C/min to -40°C.xa0 If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.xa0 At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximatelyxa0-1°C/min.)
7. Store in either the vapor or liquid phase of a nitrogen refrigerator.
8. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.xa0 Do not agitate the ampule.xa0 Do not leave ampule in water bath after it is thawed.
9. Remove the vial from the water bath immediately after thawing.xa0 Aseptically transfer the contents of the ampule into 5 mL of fresh ATCC medium 2861.xa0
10. Incubate the tube on a 15^° horizontal slant with the cap screwed on loosely (loosened one half turn) at 42°C under constant light.
11. Maintain as described above. xa0

Ohta N, et al. Structure and organization of the mitochondrial genome of the unicellular red alga Cyanidioschyzon merolae deduced from the complete nucleotide sequence. Nucleic Acids Res. 26(22): 5190-5198, 1998. PubMed: 9801318

Ohta N, et al. Complete sequence and analysis of the plastid genome of the unicellular red alga Cyanidioschyzon merolae. DNA Res. 10(2): 67-77, 2003. PubMed: 12755171

Matsuzaki M, et al. Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D. Nature 428(6983): 653-657, 2004. PubMed: 15071595

Nozaki H, et al. A 100%-complete sequence reveals unusually simple genomic features in the hot-spring red alga Cyanidioschyzon merolae. BMC Biol. 5: 28, 2007. PubMed: 17623057