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微信小章| 产品名称： | pMyc3Pst |
|---|---|
| 商品货号： | TS196170 |
| Designations： | pMyc3Pst |
| GenBank Number： | J02019 |
| Species： | avian myelocytomatosis virus (type C) |
| Depositors： | J Bishop |
| Vector： | Construct size (kb): 5.800000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 1.5 Gene product: avian myelocytomatosis viral (v-myc) oncogene homolog( avian myelocytomatosis viral (v-myc) oncogene homolog, proto-oncogene c-myc) MYC |
| Insert Size (kb)： | 1.5 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--4.1, 1.5; EcoRI--5.6; HindIII--5.6; BamHI--5.6. Encodes from approximately amino acid 52 of myc portion of gag-myc polyprotein into region of homology with Pr-C RSV genome (1581), i.e. 158-1676. env sequences are from 1581-1676; region 450-700 is very GC-rich (GC/ATGC = 0.84). |
| References： | Kochevar DT, et al. Low level amplification of c-sis and c-myc in a spontaneous osteosarcoma model. Cancer Lett. 53: 213-222, 1990. PubMed: 2208081 Vennstrom B, et al. Molecular cloning of the avian myelocytomatosis virus genome and recovery of infectious virus by transfection of chicken cells. J. Virol. 39: 625-631, 1981. PubMed: 6268847 Alitalo K, et al. Nucleotide sequence of the v-myc oncogene of avian retrovirus MC29. Proc. Natl. Acad. Sci. USA 80: 100-104, 1983. PubMed: 6296857 |