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| 产品名称： | pASK84 |
|---|---|
| 商品货号： | TS196187 |
| Designations： | pASK84 |
| Depositors： | A Skerra |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.5869998931884770 Vector: pASK84 (plasmid) Promoters: Promoter for expression lacUV5 Construction: pASK30, pASK37-IgG1 Marker(s):ampR Construct size (kb): 4.586999893188477 Features: leader peptide: phoA marker(s): ampR promoter for expression: lacUV5 replicon: f1 replicon: pMB1 repressor gene: lacI restriction site: HindIII restriction site: NcoI restriction site: XbaI signal peptide coding region: ompA terminator: lpp coding sequence: Ig gamma 1 constant region coding sequence: Ig kappa constant region epitope tag: hexahistidine sequence (6-His) |
| Applications： | expression vector vector permitting construction of fusion proteins vector with sequences for protein export |
| Comments： | Restriction digests of the clone give the following sizes (kb): NcoI/XbaI--4.0, 0.5; HindIII/NcoI--4.0, 0.5; EcoRI--4.6. Contains part of the hinge region of immunoglobulin gamma 1. The heavy chain and light chain variable regions may be cloned into the PstI-EcoRI-BstEII and SstI-PmlI-XhoI multiple cloning sites respectively. The other unique restriction sites (XbaI NcoI HindIII) may be used for subcloning or exchange of immunoglobulin constant regions. Immunoglobulin expression vector allowing cloning of variable region domains and efficient purification of Fab fragments via immobilized metal affinity chromatography. Contains the immunoglobulin heavy chain region, part of the hinge region and the immunoglobulin kappa constant region. The products are secreted into the periplasm where protein folding and disulfide bond formation occurs. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Skerra A. A general vector, pASK84, for cloning, bacterial production, and single-step purification of antibody Fab fragments. Gene 141: 79-84, 1994. PubMed: 8163179 Arne Skerra, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried Escherichia coli containing the plasmid. |