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| 产品名称： | cCW147 |
|---|---|
| 商品货号： | TS196230 |
| Designations： | cCW147 |
| Species： | Homo sapiens, human |
| Depositors： | C Wappenschmidt |
| Applications： | Only insert DNA should be used for nick translation. |
| Vector： | Construct size (kb): 4.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.6499999761581421 Tissue: sweat gland epithelium Gene product: DNA Segment, single copy D5S9 Alleles: A1, A2 |
| Insert Size (kb)： | 0.650 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.25, 0.7; PstI--2.55, 1.2; XhoI--3.15, 0.76; SalI--4.0; HindIII--4.0. Codominant segregation demonstrated in 2 informative families with 10 individuals. Digest with HindIII gives the following constant bands: 15, 9.4, 7.2, 4.6, 2.45, 1.35, 1.25, 1.05. EcoRI detects 10 invariant bands and an additional polymorphic band at 1.8 kb (in 10/53 persons). Only insert DNA should be used for nick translation. Enzyme(s) not detecting polymorphism: BamHI, BglII, MspI, XbaI using a panel of 9 unrelated Caucasians. |
| References： | Wappenschmidt C, et al. Human cDNA probe (cCW147) detects RFLP on chromosome 5 (D5S9). Nucleic Acids Res. 15: 2399, 1987. PubMed: 2882474 |