| 产品名称: | Chlamydophila pecorum (Fukushi and Hirai) Everett et al. |
|---|---|
| 商品货号: | TS196328 |
| Classification: | Chlamydiaceae |
| Agent: | Chlamydophila pecorum (Fukushi and Hirai) Everett et al. |
| Strain: | Ovine Polyarthritis strain IPA (Iowa polyarthritis) |
| Biosafety Level: | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format: | frozen 1 mL per vial |
| Storage Conditions: | -70°C or colder |
| Comments: | FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth. Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus. |
| Effect on Host: | CPE, intracellular inclusion bodies. |
| Recommended Host: | McCoy cells (ATCC CRL-1696) |
| Growth Conditions: | Temperature: 37°C Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent. Incubation: 48 to 72 hours, a 5% CO2 in air atmosphere is recommended. |
| Name of Depositor: | LA Page, ATCC |
| Source: | Derived from existing strain (This is a tissue culture adapted version of ATCC® VR-629™. ATCC® VR-629™ was isolated from sheeps joint fluid in Iowa.) |
| References: | Page LA, Cutlip RC. Chlamydial polyarthritis in Iowa lambs. Iowa Vet. 39: 10-18, 1968. |