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| 产品名称： | pGreenTIR |
|---|---|
| 商品货号： | TS196343 |
| Designations： | pGreenTIR |
| Depositors： | W Miller, S Lindow |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.4839999675750730 Vector: pGreenTIR (plasmid) Promoters: Promoter lac Construction: pUC1813 Marker(s):ampR Construct size (kb): 3.483999967575073 Features: marker(s): ampR operator: lac promoter: lac replicon: pMB1 MCS: EcoRI...HindIII MCS: HindIII...EcoRI ribosome-binding site: Shine-Dalgarno (SD) site translational enhancer: from the phage T7 gene10 coding sequence: gfp |
| Applications： | contains easily purifiable cassette(s) for construction |
| Comments： | The gfp gene, along with the translation initiation region (TIR) can be excised with one of eight restriction enzymes (HindIII, PstI, SalI, XbaI, BamHI, SmaI, SacI or EcoRI). A green fluorescent protein (GFP) cloning cassette vector designed specifically for use in the construction of prokaryotic transcriptional fusion. The gfp allele in pGreenTIR contains both the F64L and S65T mutations that increase protein solubility and cause a
ed-shift in the excitation maximum from 395 nm to 490 nm. The vector was constructed by cloning a mutant GFP gene into the EcoRI site of pUC1813. The resulting construct was mutagenized via PCR to 1) restore the 5 end of the gene to wild-type, 2) incorporate an upstream translational enhancer and 3) change the Shine-Delgarno region (and the surrounding bp) to consensus. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Miller WG, Lindow SE. An improved GFP cloning cassette designed for prokaryotic transcriptional fusion. Gene 191: 149-153, 1997. PubMed: 9218713 |
| Shipped： | frozen |
| Shipping Information： | xa0Frozen glycerol stock of E. coli containing the plasmid. |