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微信小章| 产品名称： | Human rhinovirus 1B |
|---|---|
| 商品货号： | TS196354 |
| Classification： | Picornaviridae, Enterovirus, Human rhinovirus A |
| Agent： | Human rhinovirus 1B |
| Strain： | B632 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Genome Sequenced Strain： | Human rhinovirus 1B, complete genome |
| Comments： | Strain B632 is the prototype strain for Human rhinovirus 1B. |
| Effect on Host： | CPE, rounding and sloughing |
| Recommended Host： | H1HeLa (ATCC® CRL-1958x99) |
| Growth Conditions： | Temperature: 33°C Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere rocking continuously. End adsorption by adding virus growth medium Incubation: 2-7 days |
| Name of Depositor： | National Institute of Allergy and Infectious Diseases, ATCC® |
| Source： | Presumed from human throat washings. Treated ATCC® VR-1111™ to remove mycoplasma at ATCC®. |
| References： | Fenters JD, et al. Propagation of Rhinovirus on WI-38 cell monolayers in rolling bottles. Appl. Microbiol. 15: 1460-1464, 1967. Hughes PJ, et al. The nucleotide sequence of human rhinovirus 1B: molecular relationships within the rhinovirus genus. J. Gen. Virol. 69 (PT1): 49-58, 1988. PubMed: 2826669 Taylor-Robinson D, Tyrrell DA. Serotypes of viruses (Rhinoviruses) isolated from common colds. Lancet 1: 452-454, 1962. PubMed: 13920011 |
| Cross References： | Nucleotide (GenBank) : D00239.1 Human rhinovirus 1B, complete genome |