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| 产品名称： | Learamoeba waccamawensis Sawyer et al. |
|---|---|
| 商品货号： | TS196412 |
| Deposited As： | Rhizamoeba sp. |
| Strain Designations： | LCD-2B |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | sediment from drainage ditch, Lake Waccamaw, North Carolina, 1996 |
| Product Format： | frozen |
| Type Strain： | yes |
| Medium： | ATCC® Medium 711: PYB |
| Growth Conditions： | Max Temperature: 45.0°C Min Temperature: 35.0°C Duration: grown with mixed bacteria Protocol: ATCCNO: 50579 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule, aseptically transfer the material to the surface of a plate of ATCC medium 711 agar (15 x 100 mm), and spread it evenly over the plate using a sterile spread bar or cotton swab. Wrap the edge of the plate with time tape or parafilm and incubate upright at 35C. Within 5-7 days the entire surface of the plate will be covered with cysts. To subculture, rub an area of the surface containing cysts with a sterile cotton swab and then vigorously agitate the swab while it is suspended in 1 ml of sterile distilled water. Aseptically transfer the resulting suspension to the surface of a fresh plate and spread evenly. Incubate as above. Subculture every 14-28 days. This strain is shipped with a mixed bacterial flora, the composition of which is unknown. |
| Subcultivation： | Protocol: ATCCNO: 50579 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule, aseptically transfer the material to the surface of a plate of ATCC medium 711 agar (15 x 100 mm), and spread it evenly over the plate using a sterile spread bar or cotton swab. Wrap the edge of the plate with time tape or parafilm and incubate upright at 35C. Within 5-7 days the entire surface of the plate will be covered with cysts. To subculture, rub an area of the surface containing cysts with a sterile cotton swab and then vigorously agitate the swab while it is suspended in 1 ml of sterile distilled water. Aseptically transfer the resulting suspension to the surface of a fresh plate and spread evenly. Incubate as above. Subculture every 14-28 days. This strain is shipped with a mixed bacterial flora, the composition of which is unknown. |
| Name of Depositor： | TK Sawyer |
| Year of Origin： | 1996 |
| References： | Sawyer TK, et al. Learamoeba waccamawensis, n.g., n.sp. (Heterolobosea: Vahlkampfiidae), a new temperature-tolerant cyst-forming soil amoeba. J. Eukaryot. Microbiol. 45: 260-264, 1998. type strain |