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| 产品名称： | pRSVi-Rat-NFM |
|---|---|
| 商品货号： | TS196557 |
| Designations： | pRSVi-Rat-NFM |
| GenBank Number： | M18628 |
| Species： | Rattus norvegicus, rat |
| Depositors： | RK Liem |
| Vector： | Construct size (kb): 6.300000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.700000047683716 Tissue: brain Gene product: neurofilament, medium polypeptide (170 kDa)( neurofilament subunit M (NF-M), neurofilament, subunit M, neurofilament protein, middle polypeptide) Nefm |
| Insert Size (kb)： | 2.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.3; HindIII--2.7, 3.6; PstI--2.5, 2.2, 1.0, 0.3 doublet. Insert contains the following restriction sites (approximate kb from the 5 end): BclI--0.53, 2.04; BglI--0.61; PstI--0.32, 0.69, 0.98; PvuII--0.32, 0.44, 0.52, 0.97, 1.1; SacI--1.00; SphI--2.68; StuI--0.43; XhoI--0.84, 1.00, 1.65; XmnI--2.62. |
| References： | Napolitano EW, et al. Complete amino acid sequence and in vitro expression of rat NF-M, the middle molecular weight neurofilament protein. J. Neurosci. 7: 2590-2599, 1987. PubMed: 2441012 |