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微信小章| 产品名称： | Cunninghamella elegans Lendner |
|---|---|
| 商品货号： | TS196626 |
| Deposited As： | Cunninghamella elegans Lendner |
| Strain Designations： | PA-1 |
| Application： | Degrades 1-nitropyrene
Degrades acenaphthene
Degrades acridine
Degrades dibenzothiophene
Degrades fluoranthene
Degrades petroleum crude oil
Degrades phenanthrene
Metabolizes 1-fluoronaphthalene
Metabolizes 9,10-dimethylanthracene
Metabolizes 9-hydroxymethylanthracene
Metabolizes 9-methylanthracene
Metabolizes fluorene
Oxidizes dibenzothiophene
Oxidizes isoquinoline
Oxidizes quinoline
Produces 3-hydroxywarfarin
Produces acridine trans-1,2-dihydrodiols
Produces trans-dihydrodiols
Transforms 1-nitrobenzoepyrene
Transforms benzaanthracene
Transforms brompheniramine
Transforms chlorpheniramine
Transforms pheniramine
Degrades phenanthrene to trans-dihydrodiols
Degrades acridine to acridine trans-l,2-dihydrodiol and 2-hydroxyacridine
Detoxification of 1-nitropyrene
Oxidizes dibenzothiophene to dibenzothiophene sulphoxide and dibenzothiophene sulphone
Transformation of benzaanthracene to trans-dihydrodiols |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Preceptrol®： | no |
| Genome Sequenced Strain： | Yes |
| Comments： | Genome sequencing strain (Concordia University, Canada). |
| Morphology： | After 10 days colonies spreading rapidly, at first white, later with dark spots of conidia, becoming gray, conidiophores erect, with verticillate or solitary branches; vesicles subglobose to pyriform, verrucose or smooth, conidia globose. 7-11 µm, or ovoid to ellipsoidal, 6-10 x 9-13 µm, smooth, verruculose, or shortly echinulate, hyaline, or with granular contents, brownish in mass. |
| Medium： | ATCC® Medium 200: YM agar or YM broth ATCC® Medium 323: Malt agar medium ATCC® Medium 336: Potato dextrose agar (PDA) |
| Growth Conditions： | Temperature: 24°C to 26°C Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTTATTCGGTCATTGGTTTTTATTCAAAAACCTTTGGCTTTAAATCATCCACAGTGTGGGAAATGTCTTCTAACGCTTGTGCCTGGTTCAGTCTAGTGCTGCCACTTGAGTTTACTCTTGGGTCAAGGGACCTTTGGGTAGTTTGTTCATTCGTGAGCAACCTCTTGTAACGGGGATAAGATTAATTTTATTATACTAAATTTTACTGAACTGATAGACCATAAATCTATGGTTGTTTTTTATTATAAACAAAAAAACAACTTTCAGCAATGGATCTCTCGGCTTTCGTATCGATGAAGAACGCAGCAAATCGCGATATGTAATGTGATCTGCCTATAGTGAATCATCAAATCTTTGAACGCATCTTGCACCTTATGGTATTCCATAAGGTACGTCTGTTTCAGTACCACTAGTAAATCTCCCCTCCACCTTGGTGGTTTAAAAGGAAGAGATAAATTATTACTGGTTCTGGTGATTCTTGATTTATTAAGAATTACTCTCGACCTAAATATAAGGCTCGACTTTTTTATTAGATCTCGCATCTGGTAAAACCTAGTCGGCTTTAATAGGATTTATTTCTTATTAGGTTTATAGCCATCATTTTACTTTTAAATCTTGGCCTGAAATCAGATGGGACTACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAAATAACAATGATTCCCCTAGTAACGGCGAGTGAAGAGGGAAAAGCTCAAAGTTGGAACCTGGTGGGCATAGCTCACCCGGATTGTAAACTAAAGTTTTTGAGTCGTTTAGTCAGCCAGGTAAATAAGTCCTCTGGAAAGGGGCGACATAGAGGGTGAAATCCCCGTCTTTGGCCTGAGTTTTGGTTAGGCGTTTGGCTTGGAAACGAAGAGTCAGGTTGTTTGGGAATGCAGCCTAAAATGGGAGGTAAATCTCTCCTAAAGCTAAATATTGACGAAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGAAAAGAGGGTCAAAAAGTACGTGAAATTGCTGAAAGGGAACCGTATGAAATCAGACCTACTGGTAGGTAATCAATCTTTCCCTTGGGAAGGATGCACTTGCCTGCTATGTATGCCAGCGACATTTTGGTTGGGAGGAAAAAAATAGAAGGAATGTAGCCTAGGCTTCGGTTTAGGTGTTATAGACTTTTATAAAATACTCTCGGCTGGAATGAGGAACGCAGCAAACCGTAAGGCGAAGATTCTAGTCGCTTGGGGGGAATAATTAGAGAATTTCTGCTTCGGGTGGTGCTTTGATTATTACTTTCAACTCGGTTGGAGTTCTTTAATTTGCTTAGGTTGTTGGCTTAATGATTTATATGAC |
| Name of Depositor： | JJ Perry |
| Isolation： | Estuarine mud |
| Cross References： | Nucleotide (GenBank) : Y17298 nucleotide and amino acid sequences of mRNA for enolase Nucleotide (GenBank) : AF195659 Cunninghamella elegans NADPH-dependent cytochrome P450 Nucleotide (GenBank) : Y17297 Cunninghamella elegans mRNA for 6-phosphogluconate dehydrogenase. |
| References： | Perry JJ, Cerniglia CE. Effect of substrate on the fatty acid composition of hydrocarbon-utilizing filamentous fungi. J. Bacteriol. 118: 844-847, 1974. PubMed: 4829928 Cerniglia CE, et al. Fungal metabolism and detoxification of the nitropolycyclic aromatic hydrocarbon 1-nitropyrene. Appl. Environ. Microbiol. 50: 649-655, 1985. PubMed: 3907498 Crawford DL, Gupta RK. Oxidation of dibenzothiophene by Cunninghamella elegans. Curr. Microbiol. 21: 229-231, 1990. Cerniglia CE, et al. Effects of a fluoro substituent on the fungal metabolism of 1- fluoronaphthalene. Appl. Environ. Microbiol. 48: 294-300, 1984. PubMed: 6486779 Cerniglia CE, et al. Stereoselective fungal metabolism of methylated anthracenes. Appl. Environ. Microbiol. 56: 661-668, 1990. PubMed: 2317041 Pothuluri JV, et al. Fungal metabolism and detoxification of fluoranthene. Appl. Environ. Microbiol. 58: 937-941, 1992. PubMed: 1575497 Pothuluri JV, et al. Fungal metabolism of acenaphthene by Cunninghamella elegans. Appl. Environ. Microbiol. 58: 3654-3659, 1992. PubMed: 1482186 Pothuluri JV, et al. Biotransformation of fluorene by the fungus Cunninghamella elegans. Appl. Environ. Microbiol. 59: 1977-1980, 1993. PubMed: 8328814 Sutherland JB, et al. Enantiomeric composition of the trans-dihydrodiols produced from phenanthrene by fungi. Appl. Environ. Microbiol. 59: 2145-2149, 1993. Cerniglia CE, et al. Metabolism of benzaanthracene by the filamentous fungus Cunninghamella elegans. Appl. Environ. Microbiol. 60: 3931-3938, 1994. PubMed: 7993083 Wong YW, Davis PJ. Microbial models of mammalian metabolism: production of 3- hydroxywarfarin, a new metabolite of warfarin using Cunninghamella elegans. J. Pharm. Sci. 80: 305-308, 1991. PubMed: 1865328 Schlenk D, et al. P450 catalysed S-oxidation of dibenzothiophene by Cunninghamella elegans. Xenobiotica 24: 1077-1083, 1994. PubMed: 7701849 Hansen EB Jr., et al. Fungal transformations of antihistamines: metabolism of brompheniramine, chlorpheniramine, and pheniramine to N-oxide and N- demethylated metabolites by the fungus Cunninghamella elegans. Xenobiotica 25: 1081-1092, 1995. PubMed: 8578764 Pothuluri JV, et al. Biotransformation of 1-nitrobenzoepyrene by the fungus Cunninghamella elegans. J. Ind. Microbiol. Biotechnol. 22: 52-57, 1999. Wang RF, et al. Cloning, sequencing, and expression of the gene encoding enolase from Cunninghamella elegans. Mycol. Res. 104: 175-179, 2000. Cerniglia CE, Perry JJ. Crude oil degradation by microorganisms isolated from the marine environment. Z. Allg. Mikrobiol. 13: 299-306, 1973. PubMed: 4797813 Sutherland JB, et al. Identification of metabolites produced from acridine by Cunninghamella elegans. Mycologia 86: 117-120, 1994. Sutherland JB, et al. N-Oxidation of quinoline and isoquinoline by Cunninghamella elegans. Exp. Mycol. 18: 271-274, 1994. Kim YH, et al. Decolorization of malachite green by cytochrome c in the mitochondria of the fungus Cunninghamella elegans. Arch Biochem Biophys 494: 159-165, 2010. PubMed: 19944668 |