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| 产品名称： | YEp356R plasmid in E. coli |
|---|---|
| 商品货号： | TS196731 |
| Designations： | YEp356R plasmid in E. coli |
| Depositors： | CJ Lusty |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 |
| Vector Information： | Size (kb): 8.00xa0
Vector: YEp356R (plasmid)
Promoters: Promoter none
Construction: YEp356 (ATCC® 37731™), pUC18
Marker(s): URA3, ampR
Polylinker sites:xa0HindIII SphI PstI SalI XbaI BamHI SmaI KpnI SacI EcoRI
Possible host: Escherichia coli MC1061 (delta lacZ)
Features:
insert detection: lacZ marker(s): ampR, URA3 promoter: none replicon: pMB1, 2 micron Vector type: plasmid |
| Applications： | YE-type (episomal) shuttle vector promoter-cloning vector shuttle vector |
| Comments： | This is one of 3 promoter-cloning, YE-type shuttle vectors (ATCC® No. 37737-37739™) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.xa0 The sequence and reading frame of the multiple cloning sequence is: 5’ AAGCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATC CCC GGG TAC CGA GCT CGA ATT CCC AGC TTC GAT CCC 3’ from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase. Cloning into the HindIII, SphI, PstI SalI or XbaI sites leads to a TAG stop codon within the downstream XbaI site of the multiple cloning region. The cleavage position in the reading frame for cloning sites is (where 3= between triplets): HindIII-2; SphI-3; PstI-3; SalI-2; XbaI-2; BamHI-2; SmaI-3; KpnI-3; SacI-3; EcoRI-2. The SacI site is not unique. Restriction digests of the clone give the following sizes (kb): HindIII--8.0; EcoRI--8.0; BamHI--8.0; PstI--8.0; SalI--8.0. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915 |
| Shipped： | freeze-dried |
| Shipping Information： | in E. coli containing the plasmid |