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| 产品名称： | pK184 |
|---|---|
| 商品货号： | TS196745 |
| Designations： | pK184 |
| Depositors： | RK Holmes |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.4319999217987060 Vector: pK184 (plasmid) Promoters: Promoter lac Construction: pK18, pACYC184 Marker(s):kanR Construct size (kb): 2.431999921798706 Features: insert detection: lacZ marker(s): kanR promoter: lac replicon: p15A terminator: none |
| Applications： | A general purpose plasmid vector for use in complementation analysis with vectors having a ColE1 or pMB1 origin of replication. |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--2.4; BamHI--2.4; EcoRI--2.4; XbaI--1.45, 0.95. A general purpose plasmid vector for use in complementation analysis with vectors having a ColE1 or pMB1 origin of replication. Constructed by M.G. Jobling. pK184 consists of bases 2092-2661/0-1177 of pK18 and bases 1520-836 of pACYC184. There are 2 XbaI sites in the plasmid. pK184 (TS196745) and pK194 (ATCC 37767) have the multiple cloning sites in opposite orientations, interrupting the lacZalpha coding sequence. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Jobling MG, Holmes RK. Construction of vectors with the p15a replicon, kanamycin resistance, inducible lacZalpha and pUC18 or pUC19 multiple cloning sites. Nucleic Acids Res. 18: 5315-5316, 1990. PubMed: 2402474 Randall K Holmes, personal communication |
| Shipped： | freeze-dried |