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| 产品名称： | h628 partial cDNA |
|---|---|
| 商品货号： | TS196777 |
| Designations： | h628 partial cDNA |
| GenBank Number： | U29366 Z50115 |
| Species： | Homo sapiens, human |
| Depositors： | P Malherbe |
| Vector： | Construct size (kb): 5.349999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBC/CMV Intact vector size: 4.700 Type of vector: plasmid Vector end: SmaI Vector end: SmaI Cloning sites: ClaI EcoRI BamHI XbaI SalI PstI HindIII Polylinker sites: ClaI EcoRI BamHI XbaI SalI PstI HindIII Host range: eukaryotic cells; Escherichia coli Features (with orientation and position when available): replicon: pMB1 promoter for expression: CMV, -> other: rat preproinsulin II (rI2) intron, -> terminator: SV40 polyadenylation signal, -> marker(s): ampR replicon: SV40 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: THOP1 Gene name: thimet oligopeptidase 1 Contains complete coding sequence?: N Tissue: neuroblastoma cell line IMR32 Type of DNA: cDNA Insert end: Modification: blunted Insert end: Modification: blunted Insert size (kb): 0.628 Cross references: DNA Seq. Acc.: U29366 Nucleotides 1-628 of the insert correspond to nucleotides 345-973 of U29366. DNA Seq. Acc.: Z50115 Nucleotides 1-628 of the insert correspond to nucleotides 385-1013 of Z50115. Insert lengths(kb): 0.628000020980835 Tissue: neuroblastoma cell line IMR32 Gene product: thimet oligopeptidase 1(Thimet oligopeptidase) THOP1 |
| Insert Size (kb)： | 0.628 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--4.6, 0.65; EcoRI--5.3; KpnI--4.7, 0.35. Insert contains the following restriction sites (approximate kb from the 5 end): KpnI--0.16; StuI--0.47. Corresponds to part of the coding region, which is nt 128-2197 of GenBank Z50115. |
| References： | Thompson A, et al. Cloning and functional expression of a metalloendopeptidase from human brain with the ability to cleave a beta-APP substrate peptide. Biochem. Biophys. Res. Commun. 213: 66-73, 1995. PubMed: 7639763 Parichehr Malherbe, personal communication |