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pAcMP1/PKCbetaII

货号 TS196834
中文名称 null
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产品名称: pAcMP1/PKCbetaII
商品货号: TS196834
Designations: pAcMP1/PKCbetaII
Species: Homo sapiens, human
Vector:
Construct size (kb): 12.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pAcMP1
Intact vector size: 10.052
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites:
Host range: insect cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: basic protein
replicon: pMB1
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PRKCB2
Other gene symbols: PKCB; PRKCB
Gene name: protein kinase C, beta 2
Contains complete coding sequence?: Y
Tissue: brain, temporal cortex
Type of DNA: cDNA
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 2.0
Cross references:
Insert lengths(kb): 2.0
Tissue: brain, temporal cortex
Gene product: protein kinase C, beta 2(protein kinase C, beta II) PKCB
Insert Size (kb): 2.0
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI--10.0, 2.0; EcoRI--6.6, 3.0, 2.6; HindIII--10.0, 1.1, 1.0; PstI--11.0, 1.3; PvuII--3.8 (doublet), 2.4, 1.5, 0.6.
The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--1.96; EcoRV--1.78; HindIII--1.86; NcoI--1.23; NotI--0.48; PstI--0.15, 1.34; PvuII--0.69; SacI--1.10.
Contains the complete coding sequence only (nt 65-2086 of GenBank M13975).
References:

Hocevar BA, et al. Identification of protein kinase C (PKC) phosphorylation sites on human lamin B. J. Biol. Chem. 268: 7545-7552, 1993. PubMed: 8463284

David J Burns, personal communication