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| 产品名称： | pHG50 |
|---|---|
| 商品货号： | TS196880 |
| Designations： | pHG50 |
| Species： | Bos taurus, cow |
| Depositors： | Bio-Technology General Corporation, JP White, Bio-Technology General Corporation |
| Applications： | A HpaII fragment from pSK434 (ATCC 39784) containing lambda cI434 repressor was inserted in the ClaI site of pGH44 (ATCC 39806) to produce pHG50. |
| Vector： | Construct size (kb): 6.800000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pHG44 Intact vector size: 4.600 Type of vector: plasmid Vector end: HindIII Vector end: NdeI Cloning sites: HindIII NdeI Polylinker sites: Construction: pPS1, pRO 12 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none marker(s): ampR promoter: lambdaPL terminator: T1T2 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: bovine Gene symbol: GH Gene name: growth hormone Contains complete coding sequence?: U Chromosome: 19; Localization: 19 Type of DNA: cDNA Insert end: PstI Insert end: EcoRI Insert size (kb): 1.2 Cross references: Insert lengths(kb): 1.200000047683716 Gene product: growth hormone GH |
| Insert Size (kb)： | 1.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): NdeI/HindIII--2.6, 2.3, 1.1, 0.8; PstI--4.4, 2.4; IcoRI--6.8; HindIII--4.9, 1.9; NdeI--3.4, 3.4. A HpaII fragment from pSK434 (ATCC 39784) containing lambda cI434 repressor was inserted in the ClaI site of pGH44 (ATCC 39806) to produce pHG50. |
| References： | Hartman JR, et al. Method for producing enzymatically active eucaryotic SOD in bacteria. US Patent 4,742,004 dated May 3 1988 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |