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| 产品名称： | pJS133 |
|---|---|
| 商品货号： | TS196918 |
| Designations： | pJS133 |
| Depositors： | A Shiau |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.4000000953674320 Vector: pJS133 (plasmid) Promoters: Promoter lac Construction: CAT (from pKK232-8), pUC18 Marker(s):ampR,cmlR Construct size (kb): 3.400000095367432 Features: insert detection: lacZ marker(s): ampR, cmlR promoter: lac replicon: pMB1 |
| Applications： | contains easily purifiable cassette(s) for construction |
| Comments： | There is an EcoRI site within the CAT gene. Restriction digests of the clone give the following sizes (kb): XbaI--3.5; HindIII--2.75, 0.75. Plasmid with a promoterless CAT (chloramphenicol acetyltransferase) cassette that can be excised for use in genetic constructions. CAT expression reflects transcriptional activity only; translation stops in all 3 reading frames. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Shiau A, Smith JM. Improved cat gene cassette for promoter analysis and genetic constructions. Gene 67: 295-299, 1988. PubMed: 3169577 |
| Shipped： | freeze-dried |