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| 产品名称： | HEFBE18 |
|---|---|
| 商品货号： | TS196944 |
| Designations： | HEFBE18 |
| GenBank Number： | M85320 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 2.95799994468689 DESCRIPTION OF VECTOR COMPONENT: Name of vector: lafmid BA Intact vector size: 3.153 Type of vector: phagemid Vector end: HindIII Vector end: NotI Cloning sites: HindIII, BamHI, NotI, EcoRI Polylinker sites: HindIII, BamHI, NotI, EcoRI Other unique sites: ClaI, XmnI, ScaI, ApaLI Construction: pGEM Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, insert detection: lacZ, primer site: M13 universal, -> MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098 primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: D0S13E Genomic copy number: unique Gene name: DNA Segment, single copy, expressed Contains complete coding sequence?: U Sequence homology: Homo sapiens orthodenticle Tissue: fetal brain (from Bento Soares) Type of DNA: cDNA Insert end: Insert end: Insert size (kb): Cross references: DNA Seq. Acc.: M85320; EST: EST01828; Primer: M13 Forward Tissue: fetal brain (from Bento Soares) Gene product: orthodenticle Otd |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.45, 1.2; HindIII--5.0, 1.65; PstI--6.4, 0.25; BamHI--6.65. EST01828 includes sequences that translate into homeodomains, with the most similarity to Drosophila otd. It also includes a splice acceptor sequence that begins where the sequence of EST1828 diverges from otd. EST01828 matches PIR accession A35912 with 52.77% identity and 75% similarity over the sequence range: 242-135. Isolated from a directional library constructed by B. Soares, Columbia University. First strand synthesis was primed with an oligo(dT)-NotI primer. Much of the priming occurred by the NotI portion of the primer, so although the clones are directional, the NotI site is not necessarily the 3 end of the message. This sequence record is for Drosophila melanogaster orthodenticle (Otd). |
| References： | Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749 Burglin TR, Barnes TM. Introns in sequence tags. Nature 357: 367-368, 1992. PubMed: 1350660 J C Venter, personal communication |