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| 产品名称： | pUCSV40-T302-1 molecular clone of Simian virus 40, SV-40 |
|---|---|
| 商品货号： | TS196966 |
| Designations： | pUCSV40-T302-1 molecular clone of Simian virus 40, SV-40 |
| Species： | Simian virus 40, Simian virus 40 |
| Depositors： | JA Lednicky, JS Butel |
| Origin： | SV40-T302-1 was isolated in CV-1 cells (ATCC CCL-70) from the brain of an SIV-infected juvenile Rhesus monkey. This is a clone of a unique brain isolate of SV40 (slowest growing SV40 natural isolate encountered at time of deposit.) |
| Isolation： | SV40-T302-1 was isolated in CV-1 cells (ATCC CCL-70) from the brain of an SIV-infected juvenile Rhesus monkey. This is a clone of a unique brain isolate of SV40 (slowest growing SV40 natural isolate encountered at time of deposit.) |
| Applications： | contains sequence |
| Vector： | Construct size (kb): 7.842000007629395 DESCRIPTION OF VECTOR COMPONENT xa0xa0xa0xa0xa0xa0xa0 Type of vector:xa0 E. coli xa0xa0xa0xa0xa0xa0xa0 Name of vector:xa0 pUC-19 xa0xa0xa0xa0xa0xa0xa0 Intact vector size (kb):xa0 2.686 kb |
| Insert： | DNA: genomic Insert lengths(kb): 5.156000137329102 Tissue: Rhesus monkey brain |
| Insert Size (kb)： | 5.156 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Description of cloned agent: Name: SV40 Strain: T302-1 Classification: Papovavirus Full length clone of SV40 strain T302 Brain tissue was minced in PBS, freeze-thawed three times to lyse the cells, then passed through a 0.45 mm filter. The filtrate was used to inoculate ATCC CV-1 cells (passage 34). The viral regulatory region of this construct is archetypal. |
| Classification： | Polyomaviridae, Polyomavirus |
| References： | Lednicky JA, et al. Natural isolates of simian virus 40 from immunocompromised monkeys display extensive genetic heterogeneity: new implications for polyomavirus disease. J. Virol. 72: 3980-3990, 1998. PubMed: 9557685 |