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| 产品名称： | U937-DC-SIGN |
|---|---|
| 商品货号： | TS197045 |
| Organism： | Homo sapiens, human |
| Tissue： | pleural effusion |
| Cell Type： | lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphocyte-like |
| Culture Properties： | suspension |
| Biosafety Level： | 2 xa0Cells contain SV-40 and CMV viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | histiocytic lymphoma |
| Age： | 37 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | Study Dengue virus infection, as Dengue virus uses DC-SIGN as an attachment factor. Useful for measuring antibody-mediated neutralization and enhancement in flow cytometry-based neutralization assays. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | The U-937-DC-SIGN cell line was developed by transducing U-937 cells with the gene for human DC SIGN protein (Dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin), a known attachment factor for Dengue viruses. The cloning vector used was pcDNA3 which contains SV-40 and CMV promoter regions. |
| Clinical Data： | 37 years
Caucasian
male |
| Antigen Expression： | dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) |
| Comments： | This cell line was developed by stably transfecting U-937 cells (ATCC® No.xa0CRL-1593.2™) with pcDNA3-DC-SIGN plasmid. |
| Complete Growth Medium： | ATCC® Medium 7318: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 to 2 x 105 viable cells/mL.xa0 Interval: Maintain cell density between 1 X 105 and 2 X 106 viable cells/mL. xa0 Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density). |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 10,12 D16S539: 12 D5S818: 12 D7S820: 9, 11 THO1: 6, 9.3 TPOX: 8,11 vWA: 14,15 |
| Name of Depositor： | A. Desilva |
| Year of Origin： | January 2008 |
| References： | Kraus A, et al. Comparison of plaque- and flow cytometry-based methods for measuring dengue virus neutralization. J. Clin. Microbiol. 45(11): 3777-3780, 2007. PubMed: 17804661 |