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| 产品名称： | pVE328 MB5331 |
|---|---|
| 商品货号： | TS197211 |
| Designations： | pVE328 MB5331 |
| Depositors： | Merck & Co., Inc. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Vector Information： | Construct size (kb): 7.5 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--7.2, 0.3; PstI--3.4, 3.25, 0.95; HindIII--7.5. pVE328 is available as ATCC 67698 and TS197211. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Cosmid shuttle vector permitting packaging both as bacteriophage lambda and TG1 virions. The order of the major features in this plasmid is: pVE1 ori - BalI - tsrR - SstI - lambda cos - lamda cos - HindIII - TG1 cos - XbaI/MCS/PstI. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | MacNeil DJ. Process for the incorporation of DNA molecules into microorgansims with methyl-specific restriction systems. US Patent 5,194,387 dated Mar 16 1993 Gewain KM, et al. Cloning genes from Streptomyces avermitilis for avermectin biosynthesis and the methods for their use. US Patent 5,252,474 dated Oct 12 1993 MacNeil DJ. Characterization of a unique methyl-specific restriction system in Streptomyces avermitilis. J. Bacteriol. 170: 5607-5612, 1988. PubMed: 3056907 |
| Shipped： | freeze-dried |