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| 产品名称： | pAS1-SNF1 |
|---|---|
| 商品货号： | TS197228 |
| Designations： | pAS1-SNF1 |
| Species： | Homo sapiens, human |
| Depositors： | SJ Elledge |
| Applications： | in another host, produces protein SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase |
| Vector： | DESCRIPTION OF VECTOR COMPONENT: Name of vector: pAS1 Intact vector size: 7.100 Type of vector: phagemid Vector end: XhoI Vector end: XhoI Cloning sites: NdeI NcoI SfiI SmaI BamHI SalI Polylinker sites: NdeI NcoI SfiI SmaI BamHI SalI Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): promoter: ADC1 (ADH), -> other: GAL4 DNA-binding domain, -> epitope tag: hemagglutinin (HA), -> restriction site: EcoRI, ->, 4315 MCS: NdeI...SalI, ->, 4395-4422 terminator: ADC1 replicon: pMB1 marker(s): ampR replicon: 2 micron marker(s): TRP1, -> replicon: f1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: SNF1 Gene name: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase Contains complete coding sequence?: U Type of DNA: cDNA Insert end: XhoI Insert end: XhoI Insert size (kb): Cross references: Gene product: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase SNF1 Target Gene: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products： | component of:ATCC 87014 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): XhoI--8.5, 1.0; EcoRI--9.5; BamHI--7.2, 2.35; HindIII--4.8, 3.0, 0.9, 0.7; BamHI/HindIII--4.8, 2.2, 0.9, 0.7, 0.5. Used as a negative control in the 2-hybrid cloning system. |