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| 产品名称： | pNF3 |
|---|---|
| 商品货号： | TS197376 |
| Designations： | pNF3 |
| Depositors： | PA Scolnik |
| Applications： | expression vector shuttle vector |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--8.2, 1.0; HindIII--8.2, 1.0. A wide range of transcription levels can be obtained by varying the nitrogen source in the medium. Transcription can be immediately stopped by the introduction of oxygen and ammonia. The spcR marker is expressed in R. capsulatus. A Rhodobacter capsulatus expression vector with transcription of the insert under the control of the nifH promoter. |
| References： | Pollock D, et al. Transcription of the rhodobacter capsulatus nifHDK operon is modulated by the nitrogen source. Construction of plasmid expression vectors based on the nifHDK promoter. Gene 65: 269-275, 1988. PubMed: 3410321 |