| 产品名称： |
Salpingoeca kvevrii Carr, Richter and Nitsche |
| 商品货号： |
TS197397 |
| Deposited As： |
Salpingoeca pyxidium Kent |
| Strain Designations： |
BSA-03190006 |
| Biosafety Level： |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： |
Salt marsh sediment, Hog Island, eastern shore of Virginia, 1999 |
| Product Format： |
frozen |
| Storage Conditions： |
Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage
Freeze-dried Cultures:
2-8°C
Live Cultures:
See Protocols section for handling information |
| Type Strain： |
no |
| Comments： |
Phylogenetic analyses of SSU rRNA, LSU rRNA, tubA, and hsp-90 |
| Medium： |
ATCC® Medium 1525: Seawater 802 medium
|
| Growth Conditions： |
Temperature: 25°C
Culture System: Xenic, grown with Klebsiella pneumoniae subsp. pneumoniae (ATCC® 700831™) and mixed bacteria |
| Cryopreservation： |
Reagents
Cryoprotective Solution
DMSO, 2.0 mL
Fresh growth medium w/o bacteria, 8.0 mL
Harvest and Preservation
- Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
- Harvest cells from a culture that is at or near peak density by filtration and centrifugation at 800 x g for 5 min.
- Adjust the concentration of cells at least 2 x 106/mL in fresh medium.
- Mix the cell preparation and the cryoprotective solution in equal portions.
- Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
- Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.)xa0xa0
- Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
- To establish a culture from the frozen state place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.
- Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate into a T-25 tissue culture flask containing 10 mL ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC®xa0700831™).
- Incubate at 25°C with the cap screwed on tightly.
- Once the culture is established, vigorously agitate the flask and aseptically transfer 0.5 mL to 10.0 mL of bacterized ATCC medium 1525.
- Follow the protocol for maintenance of culture.
|
| Name of Depositor： |
TA Nerad |
| Special Collection： |
NSF - Protistology |
| Year of Origin： |
1999 |
| References： |
Thomas A Nerad, personal communication
Carr M, et al. Molecular phylogeny of choanoflagellates, the sister group to Metazoa. Proc. Natl. Acad. Sci. USA. 105: 16641-16646, 2008. PubMed: 18922774
|
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