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| 产品名称： | pCMV9.9 |
|---|---|
| 商品货号： | TS197400 |
| Designations： | pCMV9.9 |
| GenBank Number： | D00462 |
| Species： | Cucumber mosaic virus |
| Depositors： | JL Slightom |
| Vector： | Construct size (kb): 3.940999984741211 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.254999995231628 Gene product: genome, partial |
| Insert Size (kb)： | 1.255 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the plasmid |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.6, 1.4; HindIII--3.3, 0.7; PvuII--2.4, 0.8. The insert contains the following restriction sites (approximate kb from the 5 end): PvuII--0.44; HindIII--0.60; XhoI--0.88; XmnI--1.16. The strain was originally isolated in New York State. Contains the complete coding sequence for the coat protein (nt 298-954) and the complete intergenic region. |
| Classification： | Cucumovirus |
| References： | Quemada H, et al. Nucleotide sequences of the coat protein genes and flanking regions of cucumber mosaic virus strains C and WL RNA 3. J. Gen. Virol. 70: 1065-1073, 1989. PubMed: 2732712 |