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微信小章| 产品名称： | pYNH37.3 |
|---|---|
| 商品货号： | TS197492 |
| Designations： | pYNH37.3 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | RFLPs were observed under normal hybridization stringency. |
| Vector： | Construct size (kb): 4.25 |
| Insert： | DNA: genomic Insert lengths(kb): 1.5 Gene product: DNA Segment, single copy D17S28 |
| Insert Size (kb)： | 1.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.25; HindIII--4.25; EcoRI/HindIII--2.85, 1.40; BamHI--4.25; PstI--4.25. pYNZ22 (ATCC 57574) and pYNH37 (TS197492) map to the same 30 kb SfiI fragment. The TaqI polymorphism demonstrates codominant segregation in more than 50 families. RFLPs were observed under normal hybridization stringency. Washes at 0.1X SSC, 0.1% SDS, 65C. The six-base cutters EcoRI, HindIII, BamHI, PvuII, PstI and BglII do not adequately resolve the polymorphism. |
| References： | vanTuinen P, et al. Molecular detection of microscopic and submicroscopic deletions associated with Miller-Dieker syndrome. Am. J. Hum. Genet. 43: 587-596, 1988. PubMed: 3189330 Schwartz CE, et al. Detection of submicroscopic deletions in band 17p13 in patients with the Miller-Dieker syndrome. Am. J. Hum. Genet. 43: 597-604, 1988. PubMed: 2903661 Baker SJ, et al. Chromosome 17 deletions and p53 gene mutations in colorectal carcinomas. Science 244: 217-221, 1989. PubMed: 2649981 Cogen PH, et al. Deletion mapping of the medulloblastoma locus on chromosome 17p. Genomics 8: 279-285, 1990. PubMed: 1979050 Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872 Barker D, et al. Gene for von Recklinghausen neurofibromatosis is in the pericentromeric region of chromosome 17. Science 236: 1100-1102, 1987. PubMed: 3107130 Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence pYNH37.3 on chromosome 17p D17S28. Nucleic Acids Res. 16: 782, 1988. PubMed: 2893350 Peter J OConnell, personal communication |