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| 产品名称： | EMBL3-cos-Not |
|---|---|
| 商品货号： | TS197553 |
| Designations： | EMBL3-cos-Not |
| Depositors： | NE Murray |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 48.2000007629394500 Vector: EMBL3-cos-Not (phage, lambda - replacement) Construction: EMBL3cos, polylinker from EMBL301 Construct size (kb): 48.20000076293945 Features: replicon: lambda |
| Applications： | vector for constructing genomic libraries |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--33.5, 14.5; BamHI--33.5, 14.5. By labeling the left cohesive end, which is 200 bp from cloning sites, reliable restriction maps of clones can be made from partial digests. For library construction, a double digest of the vector with BamHI + EcoRI and removal of the linker fragment reduces the number of background phage. Phosphatase treatment of digested vector eliminates background. A modified EMBL vector, with all phage coding sequences to the right of the cloning sites to facilitate restriction mapping of clones. Can be used for genomic library construction. |
| References： | Whittaker PA, et al. Enhanced recovery and restriction mapping of DNA fragments cloned in a new lambda vector. Nucleic Acids Res. 16: 6725-6736, 1988. PubMed: 2841642 Noreen E Murray, personal communication |
| Shipped： | freeze-dried |