| 产品名称: | CRI-L281 |
|---|---|
| 商品货号: | TS197593 |
| Designations: | CRI-L281 |
| Species: | Homo sapiens, human |
| Depositors: | Collaborative Research, Inc. |
| Vector: | Construct size (kb): 49.5 |
| Insert: | DNA: genomic Insert lengths(kb): 19.0 Tissue: liver Gene product: DNA Segment, single copy D7S54 Alleles: A5, A6, B1, B2, B4, A1, A3, A4, B3, B5, A2 |
| Insert Size (kb): | 19.0 |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried bacteria-free lysate |
| Comments: | Restriction digests of the clone give the following sizes (kb): HindIII-->23.0, 15.0, 2.9 (doublet); KpnI--21.0, 15.0, 9.4, 3.3, 1.4; SacI--23.0, 14.0, 4.2, 4.0, 1.9, 1.1; SmaI--23.0, 9.0, 7.0, 3.5, 2.6, 1.3, 0.6; EcoRI--20.0, 11.0, 9.4, 4.2, 3.7, 1.5, 0.4. The MspI and TaqI polymorphisms have a combined PIC value of 0.71 and a combined heterozygosity of 79%. Enzyme(s) not detecting polymorphism: BglII, BamHI (panel of 5 individuals). |
| References: | Schumm JW, et al. Identification of more than 500 RFLPs by screening random genomic clones. Am. J. Hum. Genet. 42: 143-159, 1988. PubMed: 2892396 Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638 Kazazian HH Jr., et al. Characterization of a spontaneous mutation to a B-thalassemia allele. Am. J. Hum. Genet. 38: 860-867, 1986. PubMed: 3014870 Barker D, et al. Genetic linkage map of human chromosome 7 with 63 DNA markers. Proc. Natl. Acad. Sci. USA 84: 8006-8010, 1987. PubMed: 2891136 |