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| 产品名称： | pACYC177/ET3d/yNMT |
|---|---|
| 商品货号： | TS197601 |
| Designations： | pACYC177/ET3d/yNMT |
| Depositors： | RS Haun |
| Other IDs： | Nucleotide (GenBank) : U25270 Expression cloning vector pACYC177/ET3d/yNMT, complete sequence. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli BL21(DE3) |
| Vector Information： | Size (kb): 4.9429998397827150 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pACYC177/ET3d Intact vector size: Type of vector: plasmid Vector end: NcoI Vector end: BamHI Cloning sites: NcoI, NcoI+BamHI Polylinker sites: Construction: pACYC177,pET3d Host range: Escherichia coli Features (with orientation and position when available): replicon: p15A marker(s): kanR, -> ribosome-binding site: gene 10 promoter: T7 (phi10), -> Cross references: Vector: pACYC177/ET3d/yNMT (plasmid) Promoters: Promoter for expression T7 (phi10) Construction: pACYC177,pET3d Marker(s):kanR Construct size (kb): 4.900000095367432 Features: marker(s): kanR promoter for expression: T7 (phi10) replicon: p15A coding sequence: NMT1 |
| Applications： | produces protein peptide N-myristoyl transferase glycylpeptide N-tetradecanoyltransferase |
| Comments： | Restriction digests of the clone give the following sizes (kb): NdeI--5.5; NcoI--5.5; StuI--3.9, 1.5. Expression system compatible with ColE1 plasmids, for cotransformation and production of myristoylated proteins. Introduction of the NcoI site during amplification introduced a single base substitution T->G at nt 4. Expression of this gene in the presence of 100 uM myristic acid allows myristoylation of recombinant proteins produced by a second ColE1 plasmid. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Haun RS, et al. Effect of myristoylation on GTP-dependent binding of ADP-ribosylation factor to Golgi. J. Biol. Chem. 268: 7064-7068, 1993. PubMed: 8463239 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |