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| 产品名称： | c9196 |
|---|---|
| 商品货号： | TS197701 |
| Designations： | c9196 |
| GenBank Number： | U11583 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | L Riles, MV Olson |
| Vector： | Construct size (kb): 43.20000076293945 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pHC79 Intact vector size: 6.430 Type of vector: cosmid Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI Polylinker sites: Construction: pBR322, Charon 4A Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, tetR replicon: pMB1 terminator: none Cross references: DNA Seq. Acc.: L08873 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Saccharomyces cerevisiae Gene symbol: CBP2 Gene name: intron processing factor Contains complete coding sequence?: Y Chromosome: VIII; Localization: VIII L Genome: Saccharomyces cerevisiae Gene symbol: SSB1 Gene name: single-strand nucleic acid binding protein Contains complete coding sequence?: Y Chromosome: VIII Genome: Saccharomyces cerevisiae Gene symbol: RPL4A Other gene symbols: MAK7 Gene name: ribosomal protein L7A-1 Contains complete coding sequence?: Y Chromosome: VIII; Localization: VIII L, 44 cM from CEN Genome: Saccharomyces cerevisiae Gene symbol: GUT1 Gene name: glycerol kinase Contains complete coding sequence?: Y Chromosome: VIII; Localization: VIII L Genome: Saccharomyces cerevisiae Gene symbol: RIM1 Gene name: positive regulator of meiosis Contains complete coding sequence?: Y Chromosome: VIII Type of DNA: genomic Insert end: Insert end: Insert size (kb): 36.792 Cross references: DNA Seq. Acc.: U11583 Nucleotides 1-44587 of the insert correspond to nucleotides 10483-55069 of U11583. Insert lengths(kb): 36.79199981689453 Gene product: glycerol kinase CBP2 |
| Insert Size (kb)： | 36.792 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--14.0, 5.0, 4.0 (doublet), 2.15, 1.8 (doublet), 1.5, 1.25 (triplet), 1.15 (doublet), 1.1 (doublet), 0.95 (doublet), 0.9 (doublet), 0.7 (triplet), 0.6 (doublet), 0.5. Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 4.952, 4.182, 4.166, 2.253, 1.848, 1.848, 1.512, 1.247, 1.247, 1.241, 1.163, 1.163, 1.100, 1.100, 0.948, 0.912, 0.912, 0.869, 0.729, 0.713, 0.700, 0.616, 0.574, 0.477. In an EcoRI/HindIII digest of the cosmid, the vector fragment is 13,365 kb. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 11143-47935 of the sequence of chromosome VIII. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229 Linda Riles, personal communication |