ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided inxa0Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC)xa0(Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223. Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication:xa0Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online atxa0http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.xa0

Human Material Precautionxa0
All tissues used for isolation are obtained under informed consent and conform to HIPAA standards to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome.

Cluster of differentiation 34 (CD34) is a sialomucin-family protein that is expressed on the surface of blood, endothelial, and bone marrow-derived progenitor cells. While the functions of CD34 are not well understood, there is evidence that the protein is involved in maintaining progenitor cells in an undifferentiated state.

The ATCC maintains CD34+ bone marrow-derived and CD34+ cord blood-derived cells which may be used to support physiologically relevant research in regenerative medicine, transplant studies, stem cell biology, hematology, and oncology. These pluripotent stem cells are isolated by immunomagnetic positive selection. In addition, the ATCC Cell Systems Laboratory has conducted in-depth characterization of the cells including differentiation into all three cell lineages.