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| 产品名称： | pTrec2 plasmid in E. coli |
|---|---|
| 商品货号： | TS197789 |
| Designations： | pTrec2 plasmid in E. coli |
| GenBank Number： | M77094 |
| Species： | Bos taurus, cow |
| Depositors： | S Zozulya, L Stryer |
| Vector： | Construct size (kb): 4.1 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.7 Tissue: retina Gene product: recoverin |
| Insert Size (kb)： | 0.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/NdeI--3.2, 0.78; BglII--4.1; EcoRI--3.7, 0.31; XbaI--4.1. The 5 nt of the insert (assuming it to be the first C in the NdeI site CATATG) corresponds to nt 25 in the sequence record. However the next two nt were changed by PCR from CC in the wild type cDNA to AT to create the NdeI site. Contains the complete coding sequence and 0.15 kb of the 3 untranslated region. |
| References： | Ray S, et al. Cloning, expression, and crystallization of recoverin, a calcium sensor in vision. Proc. Natl. Acad. Sci. USA 89: 5705-5709, 1992. PubMed: 1385864 Dizhoor AM, et al. Recoverin: a calcium sensitive activator of retinal rod guanylate cyclase. Science 251: 915-918, 1991. PubMed: 1672047 Sergey Zozulya, personal communication |