微信小陈
微信小章
产品简介
购买须知
| 产品名称： | YCp8phocat |
|---|---|
| 商品货号： | TS197840 |
| Designations： | YCp8phocat |
| Depositors： | H Feldmann |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.2600002288818360 Vector: YCp8phocat (plasmid) Promoters: Promoter PHO5 Construction: YRp7-32phocat (ATCC 37625), URA3, CEN6 Marker(s):TRP1,URA3,ampR Construct size (kb): 9.260000228881836 Features: marker(s): ampR, TRP1, URA3 promoter: PHO5 replicon: pMB1, ARS1 centromere: CEN6 |
| Applications： | YC-type (centromeric) shuttle vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--7.2, 2.1; BglII--6.0, 2.6; EcoRI--7.2, 1.5, 0.6. The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al. One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast. A 7285 bp BamHI/HindIII fragment of YCp8 containing URA3 and CEN6 was ligated to a 1975 bp BamHI/HindIII fragment of YRp7-32phocat (ATCC 37625). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257 |
| Shipped： | freeze-dried |