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| 产品名称： | pMOB1 |
|---|---|
| 商品货号： | TS197908 |
| Designations： | pMOB1 |
| Species： | pCU1 |
| Depositors： | HP Schweizer |
| Vector： | Construct size (kb): 4.599999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 1.5 Gene product: (IncN) origin of transfer replication oriT |
| Insert Size (kb)： | 1.5 |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): NotI--2.4, 2.0; EcoRI/BamHI--3.0, 1.5; EcoRI/HindIII--3.0, 1.5; HindIII--4.5. Available for research purposes only. Plasmid containing an oriT mobilization cassette that can be inserted into general purpose vectors (ATCC 77278, 77279) for conjugal transfer of cloned inserts into the chromosome of P. aeruginosa. The oriT-containing fragment can be excised more precisely with an EcoRI/BamHI double digest. The NotI digest excises a fragment of approximately 1.9 kb that includes short segments of the pHSS21 vector. The NotI cassette includes these features: pHSS21 sequence - EcoRI - oriT - PstI - BamHI - XbaI - SalI - PstI - SphI - HindIII - pHSS21 sequence. |
| References： | Schweizer HP. Allelic exchange in Pseudomonas aeruginosa using novel ColE1-type vectors and a family of cassettes containing a portable oriT and the counter-selectable Bacillus subtilis sacB marker. Mol. Microbiol. 6: 1195-1204, 1992. PubMed: 1588818 |