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| 产品名称： | Bovine herpesvirus 1 |
|---|---|
| 商品货号： | TS198033 |
| Classification： | Herpesviridae, Varicellovirus |
| Deposited As： | Infectious bovine rhinotracheitis |
| Agent： | Bovine herpesvirus 1 |
| Strain： | Los Angeles |
| Common Name： | Infectious bovine rhinotracheitis virus, Bovine rhinotracheitis virus |
| Applications： | Produces hemolysin but not HA. Stable at -60°C. Single antigenic group. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Produces hemolysin but not hemagluttinin. |
| Effect on Host： | CPE, rounding and sloughing |
| Recommended Host： | EBTr (NBL-4) (ATCC® CCL-44™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 3 days |
| Name of Depositor： | DG McKercher, UC Davis School of Veterinary Medicine |
| Source： | Dairy cattle, Los Angeles County, California, 1956 |
| References： | Madin SH, et al. Isolation of the infectious bovine rhinotracheitis virus. Science 124: 721-722, 1956. PubMed: 13371311 |