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| 产品名称： | pCMVADH |
|---|---|
| 商品货号： | TS198046 |
| Designations： | pCMVADH |
| Depositors： | LA Culp |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.0000000000000000 Vector: pCMVADH (plasmid) Promoters: Promoter CMV Marker(s):neoR Construct size (kb): 9.0 Features: marker(s): neoR promoter: CMV, RSV replicon: pMB1, M13, SV40, polyoma terminator: SV40 |
| Applications： | reporter construct shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--9.2; HindIII/XbaI--7.2, 1.8; SmaI--9.2; StuI--9.2. Shuttle reporter plasmid permitting visual detection of activity by histochemical staining. Presence of alcohol dehydrogenase activity is used to follow cell lineage in culture or in situ. The order of the major features in this plasmid is: CMV promoter - Drosophila ADH-s - SV40 polyadenylation signal - RSV promoter - neoR - polyoma ori - SV40 ori - pMB1 ori. |
| Media： | ATCC® Medium 1799: LB medium (ATCC medium 1065) plus 10 mcg/ml neomycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Lin WC, Culp LA. Selectable plasmid vectors with alternative and ultrasensitive histochemical marker genes. BioTechniques 11: 344-351, 1991. PubMed: 1931036 |
| Shipped： | freeze-dried |