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微信小章| 产品名称： | Acanthamoeba castellanii (Douglas) Page |
|---|---|
| 商品货号： | TS198052 |
| Strain Designations： | CCAP 1501/2g |
| Application： | Characterization of plasminogen activator Contact lens disinfectants against cysts In vitro characterization of cytopathic effect Successful immunization against keratitis characterization of Acanthamoeba polyphaga |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | human cornea, Cambridge, England, 1974 |
| Product Format： | frozen |
| Type Strain： | no |
| Comments： | In vivo and in vitro collagenolytic activity case report Chemotatic response of macrophages Susceptibility of corneas from various animal species Survival in contact lens rinse solution In vitro intercellular adherence Characterization of plasminogen activator mitochondrial DNA fingerprinting Amebostomes Antibody dependent neutrophil-mediated killing Contact lens disinfectants against cysts In vitro characterization of cytopathic effect Effect on apoptosis of tumor cells Successful immunization against keratitis characterization of Acanthamoeba polyphaga An axenic version of this strain is available as ATCC 50514 |
| Medium： | ATCC® Medium 997: Fresh water ameba medium |
| Growth Conditions： | Temperature: 25.0°C Duration: grown with Escherichia coli Protocol: ATCCNO: 30011 SPEC: This strain is distributed as a dried preparation. See the general procedures for opening a dried vial. Aseptically add 1 ml of sterile distilled water to the inner shell vial, remove the filter paper aseptically with a pair of forceps, and place it in the center of an agar plate of ATCC medium 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days. |
| Subcultivation： | Protocol: ATCCNO: 30011 SPEC: This strain is distributed as a dried preparation. See the general procedures for opening a dried vial. Aseptically add 1 ml of sterile distilled water to the inner shell vial, remove the filter paper aseptically with a pair of forceps, and place it in the center of an agar plate of ATCC medium 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days. |
| Cryopreservation： | Cryoprotective Solution DMSO xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 1.5 ml Dryls solution (or similar)xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 8.5 ml 1.xa0xa0xa0xa0 Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat. 2. xa0xa0 Harvest cells from a culture which is at or near peak density by adding 5 ml ATCC medium 5080 (Dryls solution) and washing cells into suspension.xa0 Rub the surface of the plate with a spread bar to detach adhering trophozoites. 3. xa0xa0xa0 Adjust the concentration of cells to at least 2 x 106/ml in fresh medium. 4.xa0 xa0xa0 Mix the cell preparation and the cryoprotective solution in equal portions. 5.xa0 xa0xa0 Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). 6. xa0xa0xa0 Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0 7.xa0 xa0xa0 Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator. 8.xa0 xa0xa0 To establish a culture from the frozen state place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0xa0 Immediately after thawing, aseptically transfer the contents of the ampule to the center of a fresh plate of ATCC medium 997.xa0 Distribute the material evenly over the plate using a spread bar. 9.xa0xa0xa0xa0 Wrap the entire edge of the plate with parafilm and incubate upright at 25°C. Follow the protocol for maintenance of culture. |
| Mycoplasma： | Unknown |
| Name of Depositor： | CCAP |
| Special Collection： | NCRR Contract |
| Chain of Custody： | ATCC < |
| Year of Origin： | 1974 |
| References： | Silvany RE, et al. The effect of currently available contact lens disinfection systems on Acanthamoeba castellanii and Acanthamoeba polyphaga. Ophthalmology 97: 286-290, 1990. PubMed: 2336265 Naginton J, et al. Amoebic infection of the eye. Lancet 2: 1537-1540, 1974. PubMed: 4140981 Stewart GL, et al. Chemotactic response of macrophages to Acanthamoeba castellanii antigen and antibody-dependent macrophage-mediated killing of the parasite. J. Parasitol. 78: 849-855, 1992. PubMed: 1403427 Nauheim RC, et al. Survival of Acanthamoeba in contact lens rinse solutions. Cornea 9: 290-293, 1990. PubMed: 2127739 Niederkorn JY, et al. Susceptibility of corneas from various animal species to in vitro binding and invasion by Acanthamoeba castellanii corrected published erratum appears in Invest. Ophthalmol. Vis. Sci. 33: 2577, 1992. Invest. Ophthalmol. Vis. Sci. 33: 104-112, 1992. PubMed: 1730531 He YG, et al. In vivo and in vitro collagenolytic activity of Acanthamoeba castellanii. Invest. Ophthalmol. Vis. Sci. 31: 2235-2240, 1990. PubMed: 2173683 Silvany RE, et al. Effect of contact lens preservatives on Acanthamoeba. Ophthalmology 98: 854-857, 1991. PubMed: 1866136 Ubelaker JE, et al. In vitro intercellular adherence of Acanthamoeba castellanii: A scanning and transmission electron microscopy study. Cornea 10: 299-304, 1991. PubMed: 1889215 Mitra MM, et al. Characterization of a plasminogen activator produced by Acanthamoeba castellanii. Mol. Biochem. Parasitol. 73: 157-164, 1995. PubMed: 8577323 Gautom RK, et al. Mitochondrial DNA fingerprinting of Acanthamoeba spp. isolated from clinical and environmental sources. J. Clin. Microbiol. 32: 1070-1073, 1994. PubMed: 7913095 Ubelaker JE, et al. Amebostomes on the amoeba Acanthamoeba castellanii (Acanthamoebidae: Amoebidae). Trans. Am. Microsc. Soc. 113: 211-215, 1994. Daggett PM, et al. Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microb. Ecol. 8: 371-386, 1982. Stewart GL, et al. Antibody-dependent neutrophil-mediated killing of Acanthamoeba castellanii. Int. J. Parasitol. 24: 739-742, 1994. PubMed: 7928077 Kilvington S, et al. Effect of contact lens disinfectants against Acanthamoeba cysts. Rev. Infect. Dis. 13 suppl.5: S414-S415, 1991. PubMed: 2047678 Taylor WM, et al. In vitro characterization of Acanthamoeba castellanii cytopathic effect. J. Parasitol. 81: 603-609, 1995. PubMed: 7623204 Alizadeh H, et al. Apoptosis as a mechanism of cytolysis of tumor cells by a pathogenic free-Living amoeba. Infect. Immun. 62: 1298-1303, 1994. PubMed: 8132336 Alizadeh H, et al. Successful immunization against Acanthamoeba keratitis in a pig model. Cornea 14: 180-186, 1995. PubMed: 7743802 Fritsche TR, et al. Occurrence of bacterial endosymbionts in Acanthamoeba spp. isolated from corneal and environmental specimens and contact lenses. J. Clin. Microbiol. 31: 1122-1126, 1993. PubMed: 8501212 Alizadeh H, et al. In vitro amoebicidal activity of propamidine and pentamidine isethionate against Acanthamoeba species and toxicity to corneal tissues. Cornea 16: 94-100, 1997. PubMed: 8985640 Niszl IA, et al. Cytopathogenicity of clinical and environmental Acanthamoeba isolates for two mammalian cell lines. J. Parasitol. 84: 961-967, 1998. PubMed: 9794638 Hurt M, et al. Exacerbation of Acanthamoeba keratitis in animals treated with anti-macrophage inflammatory protein 2 or antineutrophil antibodies. Infect. Immun. 69: 2988-2995, 2001. PubMed: 11292716 Alizadeh H, et al. Tear IgA and serum IgG antibodies against Acanthamoeba in patients with Acanthamoeba keratitis. Cornea 20: 622-627, 2001. PubMed: 11473164 Alves JMP, et al. Random amplified polymorphic DNA probes as a tool for the characterization of Brazilian keratitis isolates of the genus Acanthamoeba. Braz. J. Med. Biol. Res. 33: 19-26, 2000. Aksozek A, et al. Resistance of Acanthamoeba castellanii cysts to physical, chemical, and radiological conditions. J. Parasitol. 88: 621-623, 2002. PubMed: 12099437 Kennett MJ, et al. Acanthamoeba castellanii: characterization of an adhesin molecule. Exp. Parasitol. 92: 161-169, 1999. PubMed: 10403757 Wang X, Ahearn DG. Effect of bacteria on survival and growth of Acanthamoeba castellanii. Curr. Microbiol. 34: 212-215, 1997. PubMed: 9058539 |