| 产品名称: | pUC21-NotI |
|---|---|
| 商品货号: | TS198081 |
| Designations: | pUC21-NotI |
| Depositors: | DJ Stillman |
| Biosafety Level: | 1 |
| Vector Information: | Size (kb): 3.2500000000000000 Vector: pUC21-NotI (plasmid) Construction: pUC21deltaBB and pFA6b Marker(s):ampR Construct size (kb): 3.25 |
| Applications: | cloning vector |
| Comments: | Restriction digests of the clone give the following sizes (kb): PvuI--2.0, 1.2; PstI--3.25; XbaI--3.25. |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions: | Temperature: 37.0°C Protocol: (LB + amp) |
| References: | Vieira J. New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100: 189-194, 1991. PubMed: 1905257 Wach A, et al. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.. Yeast 10(13): 1793-1808, 1994. PubMed: 7747518 |
| Shipped: | freeze-dried |