宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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hMT-IIA [MT2A, p84H]

货号 TS198109
中文名称 null
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产品名称: hMT-IIA MT2A, p84H
商品货号: TS198109
Designations: hMT-IIA MT2A, p84H
GenBank Number:

J00271

Species: Homo sapiens, human
Depositors: M Karin
Vector:
Construct size (kb): 7.599999904632568
Insert:
DNA: genomic
Insert lengths(kb): 3.200000047683716
Gene product: metallothionein 2(metallothionein II) MT2
Alleles: A1, A2, B1, B2, A1, A2, B1, B2
Insert Size (kb): 3.200
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI--4.6, 2.25, 0.3; SalI--7.6; PstI--6.6, 0.8; HindIII--4.3, 3.1.
Insert includes entire coding sequence, 2 introns, 5 and 3 flanking regions. It spans about 900 bp. Expression is inducible by Cd++, Zn++, and glucocorticoids.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
References:

Richards RI, et al. Structural and functional analysis of the human metallothionein-IA gene: Differential induction by metal ions and glucocorticoids. Cell 37: 263-272, 1984. PubMed: 6327055

Karin M, Richards RI. Human metallothionein genes--primary structure of the metallothionein-II gene and a related processed gene. Nature 299: 797-802, 1982. PubMed: 7133118

Hyland VJ, et al. A 5 flanking region of the metallothionein, MT2A, gene identifies two moderately frequent RFLPs. Nucleic Acids Res. 15: 1350, 1987. PubMed: 2881275

Barbara Weiffenbach, personal communication