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| 产品名称： | pSP1 |
|---|---|
| 商品货号： | TS198143 |
| Designations： | pSP1 |
| Depositors： | G Cottarel |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.7030000686645510 Vector: pSP1 (phagemid) Promoters: Promoter T3 Construction: pRS305 (ATCC 77140) Marker(s):LEU2,ampR Construct size (kb): 6.703000068664551 Features: insert detection: lacZ marker(s): LEU2 marker(s): ampR promoter: lac, T3, T7 replicon: ars1 replicon: f1 replicon: pMB1 |
| Applications： | shuttle vector vector containing primer sites useful for sequencing vector for generating nested deletions of cloned inserts vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): KpnI--5.0, 1.8; SstI--6.7; HindIII--6.7. Shuttle vector transforming S. pombe at high efficiency. Suitable for generating nested deletions, DNA sequencing, and producing riboprobes. Permits visual detection of recombinants by lacZalpha complementation. Constructed from pRS305 by inserting a 1.2 kb EcoRI fragment containing ars1 into the AatII site. The NdeI site in ars1 was removed. The order of the major features (with directions of transcription) is: < LEU2 - f1 - < lacZ - SacI/MCS/KpnI - pMB1 ori - < ampR - ars1. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Cottarel G, et al. Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2. Curr. Genet. 23: 547-548, 1993. PubMed: 8319314 |
| Related Products： | component of:ATCC 87686 |
| Shipped： | frozen |
| Shipping Information： | Glycerol stock of E. coli containing the plasmid |