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| 产品名称： | pAR1173 Plasmid |
|---|---|
| 商品货号： | TS198234 |
| Designations： | pAR1173 Plasmid |
| GenBank Number： | J02518 |
| Species： | Escherichia coli bacteriophage T7 |
| Depositors： | Brookhaven National Laboratory, FW Studier, Brookhaven National Laboratory |
| Applications： | produces protein RNA polymerase |
| Vector： | Construct size (kb): 7.099999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: bacteriophage T7 Gene symbol: gene 1 Genomic copy number: unique Gene name: RNA polymerase Contains complete coding sequence?: U Type of DNA: genomic Insert end: Modification: BamHI linkers Insert end: Modification: BamHI linkers Insert size (kb): 2.7 Cross references: DNA Seq. Acc.: J02518 Insert lengths(kb): 2.700000047683716 Gene product: RNA polymerase gene 1 Target Gene: RNA polymerase |
| Insert Size (kb)： | 2.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Plasmid in Escherichia coli HMS 174 |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 2.8; BglII--7.2; EcoRI--7.2; PstI--7.2; HindIII--7.2. The insert extends from nucleotides 3145 to 5841 of T7 DNA. Gene 1 was sequenced from T7 wild-type DNA. The junctions between gene 1 and pBR322 in pAR1173 were sequenced. This construct expresses a low level of T7 RNA polymerase, enough to complement an amber mutant. pAR1173 was made by inserting a BglII linker into the BamHI site 5 of gene 1 in pAR1151. A BamHI site between the BglII site and gene 1 was lost, resulting in a BamHI-BglII-gene 1-BamHI construction. Sequences, such as promoters, inserted in the BglII site will remain with gene 1 as a single fragment upon BamHI digestion. |
| References： | Studier FW, et al. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. US Patent 4,952,496 dated Aug 28 1990 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |