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| 产品名称： | HHCMH17 |
|---|---|
| 商品货号： | TS198243 |
| Designations： | HHCMH17 |
| GenBank Number： | M78844 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: PRKMK1 Other gene symbols: MEK1 Gene name: protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) Contains complete coding sequence?: Y Chromosome: 12 Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205) Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.5 Cross references: DNA Seq. Acc.: L11284; EST: EST00992; THC id: THC180162 Nucleotides 1-241 of the insert correspond to nucleotides 803-1043 of L11284 with 100% identity. DNA Seq. Acc.: L11284; EST: EST00993; THC id: THC180162 Nucleotides 1...300 of the insert correspond to nucleotides 2138-1840 of L11284 with 96% identity. DNA Seq. Acc.: M78844; EST: EST00992; Primer: M13 Forward; THC id: THC180162 DNA Seq. Acc.: M78845; EST: EST00993; Primer: M13 Reverse; THC id: THC180162 Insert lengths(kb): 1.5 Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205) Gene product: polymyxin B resistance MEK1 |
| Insert Size (kb)： | 1.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI-3.0, 1.5. Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.4; BamHI--4.4; HindIII--4.4; PstI--4.4; XbaI--4.4; XhoI--4.4. EST00992 matches PIR accession A32714 with 76.18% identity and 95.23% similarity over the sequence range: 3-65. |
| References： | Korenberg JR, et al. Toward a cDNA map of the human genome. Genomics 29: 364-370, 1995. PubMed: 8666383 Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749 J C Venter, personal communication |