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| 产品名称： | Chlamydia trachomatis |
|---|---|
| 商品货号： | TS198266 |
| Classification： | Chlamydiaceae, Chlamydia |
| Deposited As： | Trachoma serotype b |
| Agent： | Chlamydia trachomatis |
| Strain： | Trachoma type B strain HAR-36 |
| Applications： | Sexually Transmitted Disease Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth. Note that activities with high potential for aerosol production require BSL 3 facilities and practices. Egg preparations may require adaptation to grow in tissue culture cells. Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus. |
| Effect on Host： | Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies |
| Recommended Host： | McCoy (ATCC® CRL-1696™) |
| Growth Conditions： | Temperature: 37°C
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent.
Incubation: Incubate 48 to 72 hours, a 5% CO2 in air atmosphere is recommended. |
| Name of Depositor： | RL Nichols |
| Special Collection： | ATCC |
| Source： | Human conjuctiva from a 12 month old male with trachoma (trachoma II follicles and papillae), Saudi Arabia. |
| References： | Bell SD, Fraser CE. Experimental trachoma in owl monkeys. Am. J. Trop. Med. Hyg. 18: 568-572, 1969. PubMed: 4978598 |