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| 产品名称： | pUC13/hGastricsin |
|---|---|
| 商品货号： | TS198285 |
| Designations： | pUC13/hGastricsin |
| GenBank Number： | U75272 |
| Species： | Homo sapiens, human |
| Depositors： | JJ Tang |
| Applications： | contains sequence pepsinogen C Preprogastricsin, progastricsin (pepsinogen C), urinary pepsinogen 1 |
| Vector： | Construct size (kb): 4.052000045776367 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.366000056266785 Tissue: stomach Gene product: pepsinogen C( Preprogastricsin, progastricsin (pepsinogen C), urinary pepsinogen 1) PGC Target Gene: pepsinogen C |
| Insert Size (kb)： | 1.366 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.4; BamHI--2.9, 1.2; BamHI/EcoRI--2.7, 1.2, 0.2. Using a 5 PGC probe (nt 50-398 of insert) 6.0 and 1.2Kb bands were detected with an SstI Southern hybridization. A 3 probe (nt 1083-1305 of insert) revealed a single 1.8Kb band. The 5 PGC probe (nt 50-398 of insert) and the 3 probe (nt 1083-1305 of insert) revealed the same 27.0Kb band on a HindIII Southern hybridization. The insert contains 50bp 5 UTR followed by the 1164bp progastricsin ORF and finally 137bp of 3UTR (excluding the polyA region). |