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微信小章| 产品名称： | p3-21 pTD3-21 |
|---|---|
| 商品货号： | TS198321 |
| Designations： | p3-21 pTD3-21 |
| Species： | Homo sapiens, human |
| Depositors： | M Lalande |
| Applications： | To resolve the largest two alleles of the TaqI RFLP, the depositor uses 0.6% agarose gels, 0.8 V/cm, run at 4C for 5 days. |
| Vector： | Construct size (kb): 6.599999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 2.200000047683716 Gene product: DNA Segment, single copy D15S10 Alleles: A1, A2, A3 |
| Insert Size (kb)： | 2.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Insert contains the following sites separated by (kb): HindIII- 0.62- BamHI- 0.12- EcoRI- 1.46- HindIII. Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 2.2; BamHI--4.7, 1.9; EcoRI--5.8, 0.8; SalI--6.6; EcoRI/SalI--3.6, 2.2, 0.8. To resolve the largest two alleles of the TaqI RFLP, the depositor uses 0.6% agarose gels, 0.8 V/cm, run at 4C for 5 days. |
| References： | Donlon TA, et al. Isolation of molecular probes associated with the chromosome 15 instability in the Prader-Willi syndrome. Proc. Natl. Acad. Sci. USA 83: 4408-4412, 1986. PubMed: 3012567 Nicholls RD, et al. Restriction fragment length polymorphisms with proximal 15q and their use in molecular cytogenetics and the Prader-Willi syndrome. Am. J. Med. Genet. 33: 66-77, 1989. PubMed: 2568752 Nicholls RD, Lalande M. Three allele TaqI RFLP for probe 3-21 D15S10 on chromosome 15q. Nucleic Acids Res. 17: 10140, 1989. PubMed: 2574845 Marc Lalande, personal communication |