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| 产品名称： | pMW1 plasmid in E. coli |
|---|---|
| 商品货号： | TS198523 |
| Designations： | pMW1 plasmid in E. coli |
| Depositors： | Amgen, Inc., F Lin, Amgen, Inc. |
| U.S. Patent： | |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli JM103 |
| Vector Information： | Size (kb): 12.00 DESCRIPTION OF VECTOR COMPONENT: Name of vector: unknown Intact vector size: Type of vector: plasmid Cloning sites: Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): marker(s): kanR, tetR Cross references: Vector: pMW1 (plasmid) Marker(s):kanR,tetR Construct size (kb): 12.0 Features: marker(s): kanR, tetR replicon: pSH1 repressor gene: cI857 |
| Applications： | contains sequence repressor protein cI857 mutation |
| Comments： | Temperature sensitive, grow at 28C. A plasmid to provide a temperature sensitive lambda cI857 repressor for regulated expression of cloned sequences from a lambda promoter. Has a pSHI replicon, compatible with colE1 vectors. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 28.0°C |
| References： | Lin FK. DNA sequences encoding erythropoietin. US Patent 4,703,008 dated Oct 27 1987 |
| Shipped： | freeze-dried |