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| 产品名称： | pSV5-dhfr |
|---|---|
| 商品货号： | TS198530 |
| Designations： | pSV5-dhfr |
| Depositors： | P Berg |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.30 Vector: pSV5-dhfr (plasmid) Promoters: Promoter SV40 Construction: pBR322, SV40, mouse Marker(s):ampR,mtxR Construct size (kb): 9.30 Features: marker(s): ampR, mtxR other: polyoma large T antigen promoter: SV40 replicon: pMB1 |
| Applications： | expression vector shuttle vector |
| Comments： | Confers selective advantage to mammalian cell lines grown in the presence of methotrexate. Selectable marker in dhfr- cell culture strains. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Subramani S, et al. Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors. Mol. Cell. Biol. 1: 854-864, 1981. PubMed: 9279398 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |