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| 产品名称： | pGIBS-PHE |
|---|---|
| 商品货号： | TS198572 |
| Designations： | pGIBS-PHE |
| GenBank Number： | M24537 |
| Species： | Bacillus subtilis (Ehrenberg) Cohn |
| Depositors： | M Follettie |
| Applications： | For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). |
| Vector： | Construct information: Total size of construct (kb): 4.2 Marker: ampR Promoter giving the sense strand: T3 Promoter giving the antisense strand: T7 Excise insert: XhoI + NotI Vector information: Vector: pBluescript II KS+ Vector type: phagemid Vector size (kb): 2.961 Markers: ampR Vector end (5’): XhoI Vector end (3’): NotI |
| Insert： | Insert size (kb): 1.32 Gene: pheB Source: Bacillus subtilis DNA: genomic Genbank accession: M24537 (Nucleotides 1-1318 of the insert correspond to nucleotides 2017-3334 of M24537) Insert end (5’): XhoI linker Insert end (3’): BamHI/poly(A)/NotI linker |
| Insert Size (kb)： | 1.320 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Frozen glycerol stock of xa0E. coli containing the phagemid |
| Comments： | For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). The insert contains nt 2017 - 3334 of Genbank M24537. The insert contains the following restriction sites (approximate kb from the 5 end): XmnI--0.36; AvaI--0.89; EcoRI--1.05. Restriction digests of the clone give the following sizes (kb): NotI--4.4; NotI/XhoI--3.0, 1.4; XhoI--4.4. |