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微信小章| 产品名称： | pTK-HSV-SCAP-T7(D443N) |
|---|---|
| 商品货号： | TS198639 |
| Designations： | pTK-HSV-SCAP-T7(D443N) |
| GenBank Number： | U67060 |
| Species： | Cricetus cricetus, hamster |
| Depositors： | JL Goldstein |
| Applications： | in another host, produces protein SREBP cleavage-activating protein |
| Vector： | Construct size (kb): 10.0 Intact vector size: 6.0 Features (with orientation and position when available): |
| Insert： | Genome: Hampster
DNA: cDNA
Insert lengths (kb): 3.8
Gene product: SREBP cleavage-activating protein SCAP*
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| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): ClaI--10.0; ClaI/NdeI--6.0, 4.0; NdeI--10.0. Insert encodes amino acids 2-1276 of the gene with a D443N mutation. |
| References： | Hua X, et al. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein. Cell 87: 415-426, 1996. PubMed: 8898195 Joseph L Goldstein, personal communication |