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| 产品名称： | Human coxsackievirus A3 |
|---|---|
| 商品货号： | TS198661 |
| Classification： | Picornaviridae, Enterovirus, Enterovirus A |
| Agent： | Human coxsackievirus A3 |
| Strain： | Olson |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Derived by adaptation from human amnion cell product ATCC® VR-163™ to RD cells (ATCC® CCL-136™). ATCC® VR-163™ and ATCC® VR-1799™ have not been compared for sequence or infectivity. |
| Effect on Host： | CPE, rounding and sloughing |
| Recommended Host： | RD (ATCC CCL-136) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-85% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 10-15 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-4 days |
| Name of Depositor： | HA Wenner, University of Kansas Medical Center; ATCC |
| Source： | Stool of 2 year old with nuchal rigidity and hamstring muscle spasm, New York, 1948 |
| References： | Sickles GM, Dalldorf G. Serologic differences amoung strains of the coxsackie group of viruses. Proc. Soc. Exp. Biol. Med. 72(1): 30, 1949. PubMed: 15391667 |